Cover: The balance of brain organoid differentiation. This drawing illustrates the remarkable self-organization capacity of cerebral organoids that allows them to recapitulate human brain development in vitro. The authors show that organizing centers induce the formation of dorsal and ventral progenitor zones separated by a defined boundary – like a yin-yang symbolizing the balance between distinct but complementary entities. Color code: Dark red = Cortical hem signaling center Red = Pax6 positive radial glia progenitors in the dorsal forebrain Magenta = TBR2 positive intermediate progenitor cells in the dorsal forebrain Yellow-green = GSX2 positive progenitors in the ventral forebrain Green = DLX2 progenitors and young neurons in the ventral forebrain Cyan = VGAT positive interneurons migrating from ventral to dorsal forebrain Purple = Excitatory neurons generated in the dorsal forebrain From Magdalena Renner, Madeline A Lancaster, Jürgen A Knoblich and
colleagues: Self-organized developmental patterning and differentiation in cerebral organoids. For details, see the Article on p 1316, also highlighted by Dias and Guillemot on p 1299. Artwork by Beata Edyta Mierzwa, Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC), Vienna, Austria; (beatascienceart.com).