Table 2. Summary of the phenotypes of Sen1Hel mutants
Sen1Hel versionATP hydrolysisRNA bindingUnwindingTerminationGrowtha
Wild type++++++++++++Normal
T1289A, R1293A+/−+/−NDND
N1413SND+/−HS
Δ1471‐1538 (ΔUP)+++++++++++ND
Δ1461‐1554 (ΔLP)++++++Lethal
L1549D+++ND+++/−ND
ΔUP, L1549DNDND++++/−ND
E1591QNDNDND
P1622LND+Lethal
T1779PND+/−+/−+/−HS
R1820Q+++NDLethal
  • ND, not done; HS, heat sensitive.

  • a Growth of yeast expressing the indicated version of Sen1 according to Chen et al (2014), except for ΔLP (see Fig EV4).